canine osteosarcoma cell line d17  (ATCC)

Journal: BMC Veterinary Research

Article Title: Metabolomic profiling and anti-tumor function of canine NK cells in tumor interaction

doi: 10.1186/s12917-026-05343-9

Figure Lengend Snippet: Metabolomic analysis of canine NK cells following 24-hour co-culture with D17 tumor cells. A Circular composition plot of identified metabolite classes. B OPLS-DA score plot (left) depicting group separation and permutation validation plot (right) assessing model robustness. Dashed ellipses represent 95% confidence intervals. C Volcano plot visualizing significantly altered metabolites (VIP > 1, P < 0.05). Red points: upregulated metabolites; Green points: downregulated metabolites. D Top affected metabolic pathways in canine NK cells when cocultured with D17 cells. E Hierarchical clustering heatmap of significantly altered metabolites enriched within the five KEGG pathways: Glycerophospholipid metabolism, Glycerolipid metabolism, Vitamin B6 metabolism, Alanine, aspartate and glutamate metabolism, and Tryptophan metabolism. Color gradient indicates relative metabolite abundance (red: high; green: low).

Article Snippet: The canine osteosarcoma cell line (D17) was purchased from the American Type Culture Collection (ATCC).

Techniques: Metabolomic, Co-Culture Assay, Biomarker Discovery

Journal: Scientific reports

Article Title: Discovery and functional characterization of canine PD-L1-targeted antibodies for evaluating antitumor efficacy in a canine osteosarcoma xenograft model.

doi: 10.1038/s41598-025-90770-1

Figure Lengend Snippet: Fig. 5. Antitumor effect of canine PD-L1 antibodies in vivo. (A) Schematic representation of the in vivo experiment. (B) Groups of mice were grafted with D17 (4×106/mouse) into their left flank and monitored for 20 days. Canine PBMC were injected intravenously after 10 days of tumor injection (2×106/mouse). #8 or #15 antibody were injected intraperitoneal with the concentration of 1 mg/mL. The tumor growth in mice treated with #8 or #15 antibody. *p < 0.05, ***p < 0.001, and ****p < 0.0001 (one-way ANOVA; n = 5 per group). (C) Tumor mass of mice. ns, not significant and *p < 0.05 (one-way ANOVA; n = 5 per group). These data are representative of three independent experiments. (D) Representative immunofluorescence images of tumors from mice treated with PBMC and canine anti-PD-L1 #8 or #15 antibody. DAPI (blue), TUNEL (green), and canine CD3 (red).

Article Snippet: The canine osteosarcoma cancer cell line D17 was purchased from American Type Culture Collection, (ATCC; Manassas, VA, USA) and cultured in Dulbecco’s Modified Eagle Medium with 4.5 g/L glucose, l-glutamine, and sodium pyruvate (Corning, NY, USA), supplemented with 10% (v/v) fetal bovine serum (FBS) (Gibco, Grand Island, NY, USA) and 1% (v/v) antibiotic–antimycotic solution (Welgene, Republic of Korea).

Techniques: In Vivo, Injection, Concentration Assay, Immunofluorescence, TUNEL Assay